Abstract Ischemia/reperfusion injury (I/R) is commonly related to acute kidney injury (AKI) and oxidative stress. Antioxidant agents are used to treat this condition. Lippia sidoides is a brazillian shrub with anti-inflammatory and anti-oxidative properties. Thus, the aim of this study is to evaluate the effect of Lippia sidoides ethanolic extract (LSEE) on in vivo and in vitro models of AKI induced by I/R. Male Wistar rats were submitted to unilateral nephrectomy and ischemia on contralateral kidney for 60 min via clamping followed by reperfusion for 48 h. They were divided into four groups: Sham, LSEE (sham-operated rats pre-treated with LSEE), I/R (rats submitted to ischemia) and I/R-LSEE (rats treated with LSEE before ischemia). Kidney tissues homogenates were used to determine stress parameters and nephrin expression. Plasma and urine samples were collected for biochemical analysis. I/R in vitro assays were evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and flow cytometry assays in Rhesus Monkey Kidney Epithelial Cells (LLC-MK2). The LSEE treatment prevented biochemical and nephrin expression alterations, as well as oxidative stress parameters. In the in vitro assay, LSEE protected against cell death, reduced the reactive oxygen species and increased mitochondrial transmembrane potential. LSEE showed biotechnological potential for a new phytomedicine as a nephroprotective agent.

As repercussões da doença renal crônica (DRC) associadas ao tratamento de hemodiálise culminam na diminuição da capacidade funcional. Os músculos da caixa torácica sofrem reduzindo da força muscular respiratória, ocasionando redução dos volumes e capacidades pulmonares. O treinamento muscular respiratório (TMR) vem sendo inserido, isoladamente, ou em combinação com exercícios aeróbios ou resistidos, visando melhorar a aptidão física destes pacientes. O objetivo desse estudo foi realizar uma revisão sistemática nas bases de dados sobre os efeitos do TMR nos pacientes dialíticos. BVS, PeDro e PubMed foram as base de dados utilizadas. Foram incluídos artigos originais que abordassem o TMR em pacientes submetidos à hemodiálise, com metodologia e protocolo de treinamento claramente descritos, publicados em língua portuguesa, inglesa ou espanhola. Dos 53 artigos encontrados, apenas 04 estudos adequaram-se aos critérios de inclusão. As variáveis incluídas nos estudos foram a força muscular respiratória, a função pulmonar, a capacidade funcional, Questionário Kidney Disease Quality of life (KDQOL-SF), além de resultados laboratoriais. Conclui-se que o TMR de pacientes dialíticos crônicos apontou melhora significativa em pelo menos um aspecto avaliado, e que em apenas um estudo não foi apontado melhora da força muscular respiratória. O TMR aumenta significativamente a força muscular respiratória dos doentes renais crônicos em hemodiálise e incrementa a função pulmonar e a capacidade funcional, com melhora dos níveis séricos de hematócritos, hemoglobina, albumina e proteína- C reativa. Aspectos da qualidade de vida, como sono, dor e energia apontam melhora quando comparado o antes e o depois da intervenção.

The repercussions of chronic kidney disease (CKD) associated with hemodialysis lead to loss of tissue and muscle strength resulting in reduced functional capacity. This loss affects the muscles of the rib cage reducing respiratory muscle strength, causing a reduction in lung volume and capacity. The respiratory muscle training (RMT) has been inserted, alone or in combination with aerobic or resistance exercise, to improve the physical fitness of these patients. The aim of this study was to conduct a systematic review in databases on the effects of respiratory muscle training in hemodialysis patients. The following BVS databases were used: PeDRO and PubMed as the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses).Original articles were included when they addressed the respiratory muscle training in patients undergoing hemodialysis, clearly described methodology and TMR, published in Portuguese, English or Spanish. Of the 53 articles found, 04 studies have complied with the inclusion criteria. The earliest publication was dated 2008, and the variables in the study included respiratory muscle strength, lung function, functional capacity, Kidney Disease Quality of life questionnaire (SF-KDQOL) and laboratory tests. In all analyzed studies, the respiratory muscle training in hemodialysis patients showed significant improvement in some variables that were assessed, and only one study did not described improvement in respiratory muscle strength. It was concluded that the respiratory muscle training significantly increases respiratory muscle strength in chronic renal failure patients on hemodialysis and increases lung function and functional capacity, with changes in serum levels of hematocrit, hemoglobin, albumin and protein-C reactive. Aspects of quality of life such as sleep, pain and energy point to improvement when compared before and after the intervention.

OBJETIVO: Investigar os fatores associados à lesão renal aguda e o prognóstico em pacientes com doença pulmonar. MÉTODOS: Foi realizado estudo prospectivo com cem pacientes consecutivos admitidos em uma unidade de terapia intensiva respiratória em Fortaleza (CE). Foram investigados fatores de risco para lesão renal aguda e mortalidade em um grupo de pacientes com doenças pulmonares. RESULTADOS: A média de idade foi de 57 anos, sendo 50% do gênero masculino. A incidência de lesão renal aguda foi maior nos pacientes com PaO2/FiO2<200 mmHg (54% versus 23,7%; p=0,02). O óbito ocorreu em 40 casos. A mortalidade no grupo com lesão renal aguda foi maior (62,8% versus 27,6%; p=0,01). A relação PaO2/FiO2<200 mmHg foi fator independente associado à lesão renal aguda (p=0,01); PEEP na admissão (OR: 3,6; IC95%: 1,3-9,6; p=0,009) e necessidade de hemodiálise (OR: 7,9; IC95%: 2,2-28,3; p=0,001) foram fatores de risco independentes para óbito. CONCLUSÃO: Houve maior mortalidade no grupo com lesão renal aguda. Mortalidade aumentada foi associada com ventilação mecânica, PEEP alta, ureia e necessidade de diálise. Estudos futuros devem ser realizados para melhor estabelecer as inter-relações entre lesão renal e pulmonar e seu impacto no prognóstico.

OBJECTIVE: To examine the factors associated with acute kidney injury and outcome in patients with lung disease. METHODS: A prospective study was conducted with 100 consecutive patients admitted to a respiratory intensive care unit in Fortaleza (CE), Brazil. The risk factors for acute kidney injury and mortality were investigated in a group of patients with lung diseases. RESULTS: The mean age of the study population was 57 years, and 50% were male. The incidence of acute kidney injury was higher in patients with PaO2/FiO2<200 mmHg (54% versus 23.7%; p=0.02). Death was observed in 40 cases and the rate of mortality of the acute kidney injury group was higher (62.8% versus 27.6%; p=0.01). The independent factor that was found to be associated with acute kidney injury was PaO2/FiO2<200 mmHg (p=0.01), and the independent risk factors for death were PEEP at admission (OR: 3.6; 95%CI: 1.3-9.6; p=0.009) and need for hemodialysis (OR: 7.9; 95%CI: 2.2-28.3; p=0.001). CONCLUSION: There was a higher mortality rate in the acute kidney injury group. Increased mortality was associated with mechanical ventilation, high PEEP, urea and need for dialysis. Further studies must be performed to better establish the relationship between kidney and lung injury and its impact on patient outcome.

BACKGROUND: There are no reports on hyponatremia and acute kidney injury (AKI) involved in the course of HIV-related toxoplasmic encephalitis (TE). The main objective of this study was to describe the occurrence of hyponatremia and its relationship with AKI and mortality in HIV-related toxoplasmic encephalitis (TE). METHODS: This was a retrospective cohort study on patients with HIV-related TE. AKI was considered only when the RIFLE (risk, injury, failure, loss, end-stage) criterion was met, after the patient was admitted. RESULTS: A total of 92 patients were included, with a mean age of 36 ± 9 years. Hyponatremia at admission was observed in 43 patients (46.7%), with AKI developing in 25 (27.1%) patients during their hospitalization. Sulfadiazine was the treatment of choice in 81% of the cases. Death occurred in 13 cases (14.1%). Low serum sodium level correlated directly with AKI and mortality. Male gender (OR 7.89, 95% CI 1.22-50.90, p = 0.03) and hyponatremia at admission (OR 4.73, 95% CI 1.22-18.30, p = 0.02) were predictors for AKI. Independent risk factors for death were AKI (OR 8.3, 95% CI 1.4-48.2, p < 0.0001) and hyponatremia (or 9.9, 95% ci 1.2-96.3, p < 0.0001). CONCLUSION: AKI and hyponatremia are frequent in TE. Hyponatremia on admission is highly associated with AKI and mortality.

A Doença Renal Crônica é um problema de saúde pública crescente no mundo. A detecção e o tratamento precoces reduziriam as altas taxas de morbimortalidade e os custos associados. Este trabalho buscou identificar o panorama do acesso ao cuidado a partir da conduta dos médicos da Atenção Primária à Saúde na linha de cuidado da doença. Aplicaram-se questionários para 62 médicos de família dos Centros de Saúde da Família do município de Fortaleza. Os achados apontam que a Taxa de Filtração Glomerular foi mensurada por apenas 8.1% dos médicos para pacientes diabéticos e 4.8% para pacientes hipertensos. Mais da metade dos médicos (51.2%) referenciariam o paciente apresentando redução leve/moderada da Taxa de Filtração Glomerular ao nível secundário. Por outro lado, 25.8% dos médicos não referenciariam o paciente com Doença Renal Crônica avançada ao especialista. A lacuna entre esses dois níveis da atenção implica em barreira de acesso ao usuário, podendo comprometer avanços no plano da integralidade. A criação de novos dispositivos no processo de trabalho torna-se urgente e o apoio matricial apresenta-se como proposta viável para a articulação das ações entre os níveis da atenção no cuidado do portador da Doença Renal Crônica ou seus fatores de risco.

Chronic kidney disease is a public health problem throughout the world. Early detection and treatment can reduce morbidity, mortality and associated expenditures. The objective of this research is to examine primary healthcare from a physician's perspective, evaluating the patient's access to other levels of healthcare. Questionnaires were given to 62 family physicians working in primary healthcare units in the city of Fortaleza. Glomerular filtration rate was measured by only 8.1% of the physicians when evaluating patients with diabetes and 4.8% when evaluating hypertensive patients. The majority of physicians (51.2%) refer patients with slight/moderate Glomerular filtration rate reduction to a nephrologist. On the other hand, 25.8% do not refer patients with advanced chronic kidney disease reduction to a specialist. The gap between these levels of healthcare creates a barrier to user access, prejudicing comprehensive healthcare. The creation of new work processes is urgently required and the matrix process is a viable option to connect these healthcare levels for the care of patients with chronic kidney disease or its main risk factors (diabetes and arterial hypertension).

A Doença Renal Crônica é um problema de saúde pública crescente no mundo. A detecção e o tratamento precoces reduziriam as altas taxas de morbimortalidade e os custos associados. Este trabalho buscou identificar o panorama do acesso ao cuidado a partir da conduta dos médicos da Atenção Primária à Saúde na linha de cuidado da doença. Aplicaram-se questionários para 62 médicos de família dos Centros de Saúde da Família do município de Fortaleza. Os achados apontam que a Taxa de Filtração Glomerular foi mensurada por apenas 8.1% dos médicos para pacientes diabéticos e 4.8% para pacientes hipertensos. Mais da metade dos médicos (51.2%) referenciariam o paciente apresentando redução leve/moderada da Taxa de Filtração Glomerular ao nível secundário. Por outro lado, 25.8% dos médicos não referenciariam o paciente com Doença Renal Crônica avançada ao especialista. A lacuna entre esses dois níveis da atenção implica em barreira de acesso ao usuário, podendo comprometer avanços no plano da integralidade. A criação de novos dispositivos no processo de trabalho torna-se urgente e o apoio matricial apresenta-se como proposta viável para a articulação das ações entre os níveis da atenção no cuidado do portador da Doença Renal Crônica ou seus fatores de risco.

Chronic kidney disease is a public health problem throughout the world. Early detection and treatment can reduce morbidity, mortality and associated expenditures. The objective of this research is to examine primary healthcare from a physician's perspective, evaluating the patient's access to other levels of healthcare. Questionnaires were given to 62 family physicians working in primary healthcare units in the city of Fortaleza. Glomerular filtration rate was measured by only 8.1% of the physicians when evaluating patients with diabetes and 4.8% when evaluating hypertensive patients. The majority of physicians (51.2%) refer patients with slight/moderate Glomerular filtration rate reduction to a nephrologist. On the other hand, 25.8% do not refer patients with advanced chronic kidney disease reduction to a specialist. The gap between these levels of healthcare creates a barrier to user access, prejudicing comprehensive healthcare. The creation of new work processes is urgently required and the matrix process is a viable option to connect these healthcare levels for the care of patients with chronic kidney disease or its main risk factors (diabetes and arterial hypertension).

CONTEXTO E OBJETIVO: Insuficiência cardíaca grave tem elevada correlação com doença renal crônica. Creatinina sérica é um indicador pobre da função renal e a estimativa da taxa de filtração glomerular (TFG) é um método acessível para avaliar a função renal. As fórmulas mais populares estimadoras da TFG são a Cockcroft-Gault (CG), a modificação de dieta simplificada em doença renal (Simplified Modification of Diet in Renal Disease, sMDRD) com quatro variáveis, e a recentemente introduzida Colaboração Epidemiológica na Doença Renal Crônica (Chronic Kidney Disease-Epidemiology Collaboration, CKD-EPI). O objetivo foi analisar a correlação entre essas três equações estimadoras da TFG em pacientes com insuficiência cardíaca grave. TIPO DE ESTUDO E LOCAL: Estudo transversal observacional em centro de referência universitário. MÉTODOS: Pacientes com insuficiência cardíaca grave em fila para transplante cardíaco tiveram a TFG estimada pelo CG, sMDRD e CKD-EPI. Essas estimativas foram analisadas pela correlação de Pearson e análise de Bland-Altman. RESULTADOS: Foram incluídos 157 pacientes: 32 (20,3%) mulheres. Creatinina sérica normal foi observada em 21,6%. TFG média de acordo com CG, sMDRD e CKD-EPI foi 70,1 ± 29,5, 70,7 ± 37,5 e 73,7 ± 30,1 ml/min/1.73 m²; P > 0,05. Coeficiente de Pearson mostrou boa correlação entre todas as fórmulas, assim como Bland-Altman. Entretanto, os pacientes apresentaram TFG < 60 ml/min mais frequentemente com a fórmula sMDRD (54,1% versus 40,2% para CG e 43,2% para CKD-EPI; P = 0,02). CONCLUSÃO: Apesar da boa correlação e concordância entre os três métodos, a fórmula do sMDRD classificou mais pacientes com TFG menor que 60 ml/min.

CONTEXT AND OBJECTIVE: Severe heart failure is highly associated with chronic kidney disease (CKD). Serum creatinine is a poor indicator of renal function and glomerular filtration rate (GFR) estimation is an accessible method for assessing renal function. The most popular formulas for GFR estimation are the Cockcroft-Gault (CG), the four-variable Simplified Modification of Diet in Renal Disease (sMDRD) and the recently introduced CKD-Epidemiology Collaboration (CKD-EPI). The objective of the study was to analyze the correlation between these three equations for estimating GFR in patients with severe heart failure. DESIGN AND SETTING: Cross-sectional observational study at a university reference center. METHODS: GFR was estimated in patients with severe heart failure who were awaiting heart transplantation, using the CG, sMDRD and CKD-EPI formulas. These estimates were analyzed using Pearson's correlation and Bland-Altman analysis. RESULTS: This study included 157 patients, of whom 32 (20.3%) were female. Normal serum creatinine concentration was observed in 21.6%. The mean GFR according to CG, sMDRD and CKD-EPI was 70.1 ± 29.5, 70.7 ± 37.5 and 73.7 ± 30.1 ml/min/1.73 m²; P > 0.05. Pearson's coefficient demonstrated good correlations between all the formulas, as did Bland-Altman. However, the patients presented GFR < 60 ml/min more frequently with the sMDRD formula (54.1% versus 40.2% for CG and 43.2% for CKD-EPI; P = 0.02). CONCLUSION: Despite the good correlation and agreement between the three methods, the sMDRD formula classified more patients as presenting GFR less than 60 ml/min.

INTRODUCTION: leptospirosis is a zoonosis of worldwide importance. The disease is endemic in Brazil. This study was conducted to describe the clinical and laboratory presentation of leptospirosis in a metropolitan city of Brazil. METHODS: this is a retrospective study including 201 consecutive patients with leptospirosis admitted to tertiary hospitals in Fortaleza, Brazil, between 1985 and 2006. All patients had clinical and epidemiological data suggestive of leptospirosis, and positive laboratorial test for leptospirosis (microscopic agglutination test, MAT, higher than 1:800). RESULTS: a total of 201 patients were included, with mean age of 38.9 ± 15.7 years; 79.1% were male. The mean length from onset of symptoms to admission was 7 ± 3 days. The main clinical signs and symptoms at admission were fever (96.5%), jaundice (94.5%), myalgia (92.5%), headache (74.6%), vomiting (71.6%) and dehydration (63.5%). Hemorrhagic manifestations were present in 35.8%. Acute kidney injury was found in 87% of the patients. Platelet count was less than 100,000/mm³ in 74.3%. Hematuria was found in 42.9%. Death occurred in 31 cases (15.4%). CONCLUSIONS: leptospirosis is a globally relevant disease with potential fatal outcome. Signs and symptoms suggestive of leptospirosis must be known by any physician in order to institute early adequate treatment to improve outcome. Early indication and daily hemodialysis seems to be beneficial in this group of patients.

OBJETIVO: Avaliar a presença de microbiota aeróbia da conjuntiva de portadores de alergia ocular e comparar a um grupo controle. MÉTODOS: Foram examinados 133 pacientes no período de abril a junho de 2001 divididos em 2 grupos. O grupo A foi composto de 63 portadores de conjuntivite alérgica (sem uso de medicação) e o grupo B de 70 pacientes do ambulatório geral (controle). Foram coletadas amostras do fundo de saco conjuntival do olho direito de todos os pacientes e o material foi semeado em meios sólidos de cultura (ágar sangue, chocolate e Sabouraud). RESULTADOS: No grupo A, 30 culturas (47,7%) foram positivas e no grupo B, 6 (8,6%). Sete bactérias foram isoladas no grupo A e 4 no B. A análise estatística revelou associação significante entre a positividade dos cultivos e conjuntivite alérgica. CONCLUSÃO: Microbiota bacteriana foi mais freqüentemente encontrada nos pacientes com alergia ocular.

PURPOSE: To evaluate de presence of conjunctival aerobic microbiota in patients with ocular allergy as compared to a control group. METHODS: One hundred and thirty-three patients were evaluated from April to June 2001 and divided into 2 groups. Sixty-three patients with allergic conjunctivitis (without medication) were in group A and 70 patients from the general outpatient clinic were in group B (control group). Samples from the conjunctival sac of the right eye were collected and cultured in solid media (blood, chocolate and Sabouraud agar). RESULTS: In group A, 30 cultures (47.7%) were positive and 6 (8.6%) in group B. Seven bacteria were isolated from group A and 4 from group B. Statistical analysis revealed significant association between positive cultures and allergic conjunctivitis. CONCLUSION: Bacterial microbiota was more frequently found in patients with ocular allergy.